METHODS: Upon continuously passaging, the androgen - independent LNCaP cell model was established.

方法: 通过连续传代培养,建立雄激素 非 依赖性前列腺癌细胞模型.

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Immunohistochemical staining with ET ? 1 antibody showed ET ? 1 in passaged EEC was positive.

传代培养的新生小牛右心室EEC的ET-1免疫组织化学显色强阳性.

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After subculture, confluence was occurred in 1 - 2 weeks.

传代培养细胞可在1～2周汇合成片.

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Results: Autogenous bone marrow - derived MSC showed active proliferative capacity in vitro in primary and passage cultures.

结果: 原代培养及传代培养显示,兔自体骨髓间充质干细胞具有活跃的增殖倍增能力.

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