DNA dissolved in double - distilled water were deposited onto gold surface and covered with 50 % glycerol - water solution.

把溶于双蒸水的脱氧核糖核酸(NA)展在金膜上,再覆盖一薄层50%油水溶液.

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The restriction digestion system consisted of 10μ L PCR products, 2.0μ L 10× Buffer, 83.350 nkat restriction enzyme and 7.5μ L ddH 2 O.

PCR产物10.0μL, 加入2.0μL10×Buffer, 83.350nkat内切酶,7.5μL双蒸水构成20μL酶 切反应体系.

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