Opportunity knocks at the door only once.

机不可失,时不再来.

《简明英汉词典》

Enroll now, we only have 30 seats for you!

现在就加入吧,机不可失, 时不再来.

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When an opportunity is neglected, it never comes back to you.

机不可失,时不再来,机会一过, 永不再来.

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Now ( in the time ) or never !

机不可失, 时不再来!

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One is not often given two bites at the cherry in the world of business.

在商界中,机不可失,时不再来.

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Dad often says that opportunity knocks only once , so one must take it when it comes.

爸爸总说机不可失、时不再来,有了机会一定要把握住.

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